No certificate holder may operate an aircraft that has a passenger seating configuration, excluding any pilot seat, of more than 19 unless there is a flight attendant crewmember on board the aircraft.
ACE rules page: Part 135 in plain English
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14 CFR · PART 135
§ 135.107 Flight attendant crewmember requirement.
Official text · Current as of Oct 19, 2026 · from eCFR
Not legal advice. This is the official text as published in the eCFR, which is updated daily but is not the legal edition. For legal purposes, use the annual Code of Federal Regulations or ask an aviation attorney.